Article 3117

Title of the article



Tarasov Sergei Sergeevich, Assistant, sub-department of botany, physiology and plant protection, Nizhny Novgorod State Agricultural Academy (97 Gagarina avenue, Nizhny Novgorod, Russia),

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Background. This work considers the influence of grain mucelium of oyster mushroom (Pleurotus ostreatus) on the oxidative modification of proteins (OMP), dismutase (SOD) in the blood plasma of the European rabbit (Orictolagus cuniculus). The aim of the study is to examine the activity of antioxidant enzymes and to explore the possibility of the influence of the grain mycelium oyster mushroom on the processes of oxidative degradation of biopolymers.
Materials and methods. The mucelium of the oyster mushroom entered the animals’ organisms with food. OMP was determined by registration of 2,4-dinitrophenylhydrazone products in the blood plasms; LPO – by registration of malon dialdehyde (MDA) and diene conjugates (DC). SOD was determined through its ability to compete with nitroblue tetrozolem of superoxide anion radicals; catalase was determined  by reducing the amount of peroxide in the sample.
Results. The author determined the influence of grain mycelium of the oyster mushroom on OMB, LPO, SOD and catalase. A decrease of OMP products was registered during the study period, which can be divided into 2 peaks: a short-term during the first 3 days – no significant change in 2,4-denitrofenilgidrazones, a longterm – recorded after 7 days from the date of the diet change. A similar pattern was observed with LPO. SOD increased its activity in the first day of the experiment, followed by a decrease. The catalase activity did not change significantly.
Conclusions. The author has shown a dependence between the level of OMP, LPO, catalase and SOD and the eaten grain mucelium, displaying a trend towards a decrease of the OMP and LPO concentration and the enzyme activity.

Key words

grain mucelium, oxidative modification of proteins (OMP), lipid peroxidation (LPO), malon dialdehyde, diene conjugates, superoxide dismutase, catalase

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